کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8645631 | 1569789 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cloning and characterization of â6/â5 fatty acyl desaturase (Fad) gene promoter in the marine teleost Siganus canaliculatus
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کلمات کلیدی
AP1GATA binding protein 2Eicosapentaenoic acid (20:5n-3)Linoleic acid (18:2n-6)Fatty acyl desaturaseNF-1LC-PUFA biosynthesisα-linolenic acid (18:3n-3)arachidonic acid (20:4n-6)NF-YDR1HNF4αSRELC-PUFAFASLNAEFATSSPPARγEPAALAC/EBP - C / EBPLC–MS - LC-MSSp1 - SP1docosahexaenoic acid (22:6n-3) - اسید داکوزاگزائنیک (22: 6n-3)Polyunsaturated fatty acids - اسید چرب اشباع نشدهfatty acid synthase - اسید چرب سنتازEssential fatty acid - اسید چرب ضروریPUFA - اسید چرب چند غیراشباعLong-chain polyunsaturated fatty acids - اسیدهای چرب اشباع نشده چای زنجیره ایFAD - بدMarine teleost - تئوستس دریاییARA - در حال حاضرDHA - دوکوساهگزائنوئیک اسیدtranscription start site - رونویسی شروع سایتTranscription factor - عامل رونویسیnuclear factor 1 - عامل هسته ای 1hepatocyte nuclear factor 4α - عامل هسته ای hepatocyte 4αNuclear factor Y - عامل هسته ای Ysterol regulatory element - عنصر نظارتی استرولUTR یا untranslated regions - منطقه ترجمه نشدهuntranslated region - منطقه غیر ترجمهCCAAT enhancer binding protein - پروتئین اتصال دهنده تقویت کننده CCAATActivated protein 1 - پروتئین فعال 1liquid chromatography coupled with tandem mass spectrometry - کروماتوگرافی مایع همراه با طیف سنجی جرمی دو طرفهGATA-2 - گاتا-2peroxisome proliferator activated receptor γ - گیرنده پروتئینزا پراکسیوم فعال γ
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The rabbitfish Siganus canaliculatus was the first marine teleost demonstrated to have the ability of biosynthesizing long-chain polyunsaturated fatty acids (LC-PUFA) from C18 PUFA precursors, and all genes encoding the key enzymes for LC-PUFA biosynthesis have been cloned and functionally characterized, which provides us a potential model to study the regulatory mechanisms of LC-PUFA biosynthesis in teleosts. As the primary step to clarify such mechanisms, present research focused on promoter analysis of gene encoding â6/â5 fatty acyl desaturase (Fad), a rate-limiting enzyme catalyzing the first step in the conversion of C18 PUFA to LC-PUFA. First, 2044â¯bp promoter sequence was cloned by genome walking, and the sequence from â456â¯bp to +51â¯bp was determined as core promoter by progressive deletion mutation. Moreover, binding sites of transcription factors (TF) such as CCAAT enhancer binding protein (C/EBP), nuclear factor 1 (NF-1), stimulatory protein 1 (Sp1), nuclear factor Y (NF-Y), activated protein 1 (AP1), sterol regulatory element (SRE), hepatocyte nuclear factor 4α (HNF4α) and peroxisome proliferator activated receptor γ (PPARγ) were identified in the core promoter by site-directed mutation and functional assays. Moreover, NF-1 and HNF4α were confirmed to interact with the core promoter region by gel shift assay and mass spectrometry. This is the first report of the promoter structure of a â6/â5 Fad in a marine teleost, and a novel discovery of NF-1 and HNF4α binding to the â6/â5 Fad promoter.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 647, 20 March 2018, Pages 174-180
Journal: Gene - Volume 647, 20 March 2018, Pages 174-180
نویسندگان
Yewei Dong, Jianhong Zhao, Junliang Chen, Shuqi Wang, Yang Liu, Qinghao Zhang, Cuihong You, Ãscar Monroig, Douglas R. Tocher, Yuanyou Li,