Genetic fusion of tetanus toxin fragment C (Hc) gene to cholera toxin subunit B (CTB) gene as a preparatory step for double vaccine production

In conclusion, construction of DNA segment encoding for the non-toxic Hc gene genetically linked to the highly immunogenic non-toxic CTB gene in one cassette was possible. This lead to the production of recombinant tetanus-cholera double protein in one expression system with a save and economic feedback. Further studies to test doses and prolongation of the vaccine are needed.