کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8646265 | 1570074 | 2018 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Extracellular matrix protein production in human adipose-derived mesenchymal stem cells on three-dimensional polycaprolactone (PCL) scaffolds responds to GDF5 or FGF2
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کلمات کلیدی
RT-qPCRMesenchymal stromal/stem cellGDF5EthD-1FGF2MSCECMThree-dimensionalPCL(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) - (3- (4،5-dimethylthiazol-2-yl) -2،5-diphenyltetrazolium bromide)MTT - MTTScapholunate - اسکاپولولاتاPolymer scaffolds - داربست پلیمرACL - رباط صلیبی جلوییAnterior cruciate ligament - رباط صلیبی جلوییMesenchymal stem/stromal cells - سلولهای ساقه / استرومال MesenchymalGrowth factors - فاکتور رشدExtracellular matrix - ماتریکس خارج سلولیEthidium homodimer - همودییمید اتیدیمPlatelet lysate - ورید لازاتPolycaprolactone - پلی کاپرولاکتونCalcein AM - کالسین AM
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The poor healing potential of intra-articular ligament injuries drives a need for the development of novel, viable 'neo-ligament' alternatives. Ex vivo approaches combining stem cell engineering, 3-dimensional biocompatible scaffold design and enhancement of biological and biomechanical functionality via the introduction of key growth factors and morphogens, represent a promising solution to ligament regeneration. We investigated growth, differentiation and extracellular matrix (ECM) protein production of human adipose-derived mesenchymal stem/stromal cells (MSCs), cultured in 5% human platelet lysate (PL) and seeded on three-dimensional polycaprolactone (PCL) scaffolds, in response to the connective-tissue related ligands fibroblast growth factor 2 (basic) (FGF2) and growth and differentiation factor-5 (GDF5). Phenotypic alterations of MSCs under different biological conditions were examined using cell viability assays, real time qPCR analysis of total RNA, as well as immunofluorescence microscopy. Phenotypic conversion of MSCs into ECM producing fibroblastic cells proceeds spontaneously in the presence of human platelet lysate. Administration of FGF2 and/or GDF5 enhances production of mRNAs for several ECM proteins including Collagen types I and III, as well as Tenomodulin (e.g., COL1A1, TNMD), but not Tenascin-C (TNC). Differences in the in situ deposition of ECM proteins Collagen type III and Tenascin-C were validated by immunofluorescence microscopy. Treatment of MSCs with FGF2 and GDF5 was not synergistic and occasionally antagonistic for ECM production. Our results suggest that GDF5 alone enhances the conversion of MSCs to fibroblastic cells possessing a phenotype consistent with that of connective-tissue fibroblasts.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene Reports - Volume 10, March 2018, Pages 149-156
Journal: Gene Reports - Volume 10, March 2018, Pages 149-156
نویسندگان
Yan Su, Janet M. Denbeigh, Emily T. Camilleri, Scott M. Riester, Joshua A. Parry, Eric R. Wagner, Michael J. Yaszemski, Allan B. Dietz, Simon M. Cool, Andre J. van Wijnen, Sanjeev Kakar,