کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
866515 | 1470976 | 2014 | 5 صفحه PDF | دانلود رایگان |
• A pyrazoline-based selective and sensitive fluorescent probe was synthesized.
• The probe was highly selective for glutathione.
• The detection limit for glutathione was 8.2×10−8 M.
• The probe can detect glutathione in cells.
A novel compound, 2-(1,5-diphenyl-4,5-dihydro-1H-pyrazol-3-yl)phenyl acrylate (probe L), was designed and synthesized as a highly sensitive and selective fluorescent probe for recognizing and detecting glutathione among cysteine, homocysteine and other amino acids. The structures of related compounds were characterized using IR, NMR and HRMS spectroscopy analysis. The probe is a non-fluorescent compound. On being mixed with glutathione in buffered EtOH:PBS=3:7 solution at pH 7.4, the probe exhibited the blue emission of the pyrazoline at 474 nm and a 83-fold enhancement in fluorescence intensity. This probe is very sensitive and displayed a linear fluorescence off–on response to glutathione with fluorometric detection limit of 8.2×10−8 M. The emission of the probe is pH independent in the physiological pH range. Live-cell imaging of HeLa cells confirmed the cell permeability of the probe and its ability to selectively discriminate GSH from Cys and Hcy in cells. The toxicity of the probe was low in cultured HeLa cells.
Journal: Biosensors and Bioelectronics - Volume 55, 15 May 2014, Pages 386–390