کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
866853 | 1470988 | 2013 | 7 صفحه PDF | دانلود رایگان |
The rising prevalence to food allergies in the past two decades, together with the fact that the only existing therapy is avoidance of allergen-containing food next to the implementation of anti-allergic drugs, urges the need for improved performance of current assays to detect potential allergens in food products. Therein, the focus has been on aptamer-based biosensors in recent years. In this paper we report for the first time the selection of aptamers against one of the most important peanut allergens, Ara h 1. Several Ara h1 DNA aptamers were selected after eight selection rounds using capillary electrophoresis (CE)-SELEX. The selected aptamers specifically recognized Ara h 1 and did not significantly bind with other proteins, including another peanut allergen Ara h 2. The dissociation constant of a best performing aptamer was in the nanomolar range as determined independently by three different approaches, which are surface plasmon resonance, fluorescence anisotropy, and capillary electrophoresis (353±82 nM, 419±63 nM, and 450±60 nM, respectively). Furthermore, the selected aptamer was used for bioassay development on a home-built fiber optic surface plasmon resonance (FO-SPR) biosensor platform for detecting Ara h 1 protein in both buffer and food matrix samples demonstrating its real potential for the development of novel, more accurate aptamer-based biosensors. In conclusion, the reported aptamer holds a great potential for the detection of Ara h 1 in both the medical field and the food sector due to its high affinity and specificity for the target protein.
► Aptamer against one of the major peanut allergens Ara h 1 is selected.
► Selected aptamer scored for high affinity and specificity toward its target.
► The aptamer against Ara h 1 is used as a bioreceptor on FO-SPR biosensor.
► The bioassay was suitable for detection of Ara h 1 protein in food matrix sample.
► Selected aptamer is proven functional beyond its selection environment.
Journal: Biosensors and Bioelectronics - Volume 43, 15 May 2013, Pages 245–251