کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
867024 1470986 2013 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Electrochemical evaluation of DNA methylation level based on the stoichiometric relationship between purine and pyrimidine bases
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Electrochemical evaluation of DNA methylation level based on the stoichiometric relationship between purine and pyrimidine bases
چکیده انگلیسی

An efficient electrochemical approach for the evaluation of DNA methylation level was proposed according to the oxidation signal of DNA bases at an overoxidized polypyrrole (PPyox) directed multiwalled carbon nanotubes (MWNTs) film modified glassy carbon electrode (GCE). The PPyox/MWNTs/GCE exhibited remarkable electrocatalytic activities towards the oxidation of DNA bases due to the advantages of wide potential window, large effective surface area, and excellent antifouling property. As a result, all purine and pyrimidine bases of guanine (G), adenine (A), thymine (T), cytosine (C) and 5-methylcytosine (5-mC) exhibited well identified oxidation peaks at the PPyox/MWNTs/GCE. The direct potential resolution between 5-mC and C was obtained to be 180 mV, which was large enough for their signal recognition and accurate detection in mixture. In particular, the signal interference from T, a great challenge in exploring DNA methylation, was successfully eliminated by an innovative strategy, which was developed based on the stoichiometric relationship between purine and pyrimidine bases in DNA molecular structure. The proposed method was effectively applied to the rapid detection of DNA methylation status in real sample within 45 min with satisfactory results.


► The method realized the rapid detection of DNA methylation information within 45 min.
► The interference from thymine was successfully eliminated by an innovative strategy.
► The peak potential of 5-methylcytosine was clearly distinguished from that of cytosine.
► The assay process was free from bisulfite conversion, hybridization and labeling steps.
► Compared with traditional methods, this approach is convenient, rapid and accurate.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 45, 15 July 2013, Pages 34–39
نویسندگان
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