کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
867063 1470986 2013 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Potentiometric aptasensing based on target-induced conformational switch of a DNA probe using a polymeric membrane silver ion-selective electrode
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Potentiometric aptasensing based on target-induced conformational switch of a DNA probe using a polymeric membrane silver ion-selective electrode
چکیده انگلیسی

In this article, we introduce a general, sensitive, facile, and label-free potentiometric assay based on metal-mediated DNA base pairs. A nucleic acid with one adenosine-5′-triphosphate (ATP) binding sequence (aptamer) in the middle and two cytosine(C)-rich sequences at the lateral portions was employed as a model. A rigid hairpin structure can be formed in the presence of Ag+ ions, in which the C residues of the spatially separated nucleotides are linked by the ions. The strong interaction between Ag+ ions and cytosines forms a stable C–Ag+–C structure, which could reduce the concentration of silver ions released from the polymeric membrane silver ion-selective electrode (ISE) at the sample–membrane interface and decrease the potential response. In the presence of its target, the aptamer (the loop sequence of the probe) binds specifically to the target via reaction incubation. Such target-binding induced aptamer conformational change prevents the formation of C–Ag+–C structure, leaving more silver ions at the sample–membrane interface, which can be detected by the silver ISE. ATP can be quantified in the range of 0.5–3.0 μM with a detection limit of 0.37 μM. The relative standard deviation for 5 μM ATP is 5.5%. For the proposed method, the combination of using ion fluxes of silver ions as modulating reagents and as signal reporters greatly simplifies the detection procedures. In addition, by changing the binding sequence in the middle of the probe, the present detection method will be able to explore new applications of ISE for the detection of a large variety of targets.


► A label-free potentiometric assay based on metal–DNA base pairs is proposed.
► Ag+ ions interacting with the C–C mismatch in the DNA probe serve as a signal reporter.
► The target-binding induced conformational change prevents the formation of C–Ag+–C structure.
► The ion fluxes of a silver ion-selective electrode are used for potentiometric aptasensing.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 45, 15 July 2013, Pages 148–151
نویسندگان
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