کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
867314 | 909780 | 2012 | 7 صفحه PDF | دانلود رایگان |
The early detection and eradication of circulating tumor cells (CTCs) play an important role in cancer metastasis management. This paper describes a new nanoparticle-enabled technique for integrated enrichment, detection and killing of CTCs by using magnetic nanoparticles and bismuth nanoparticles, X-ray fluorescence spectrometry, and X-ray radiation. The nanoparticles are modified with tumor targeting agents and conjugated with tumor cells through folate receptors over-expressed on cancer cells. A permanent micro-magnet is used to collect CTCs suspended inside a flowing medium that contains phosphate buffered saline (PBS) or whole blood. The characteristic X-ray emissions from collected bismuth nanoparticles, upon excitation with collimated X-rays, are used to detect CTCs. Results show that the method is capable of selectively detecting CTCs at concentrations ranging from 100–100,000 cells/mL in the buffer solution, with a detection limit of ∼100 CTCs/mL. Moreover, the dose of primary X-rays can be enhanced to kill the localized CTCs by radiation induced DNA damage, with minimal invasiveness, thus making in vivo personalized CTC management possible.
► Simultaneous enrichment, detection and killing of CTCs have been achieved.
► Nanoparticles are modified to target receptors overexpressed on CTCs.
► X-ray fluorescence signals from nanoparticles are used to detect CTCs.
► CTC detection ranges from 102 to 105 cells/mL in buffer.
► X-ray dose can be enhanced to kill >80% of the localized CTCs.
Journal: Biosensors and Bioelectronics - Volume 38, Issue 1, October–December 2012, Pages 348–354