کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
867327 909780 2012 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Electrochemical immunoassay for CD10 antigen using scanning electrochemical microscopy
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Electrochemical immunoassay for CD10 antigen using scanning electrochemical microscopy
چکیده انگلیسی

In the present study, a scanning electrochemical microscopic (SECM) method for imaging of antigen/antibody binding was proposed using CD10 antigen as the model. On the basis of anti-CD10 modified electrode, an electrochemical immunosensor for sensitive detection of CD10 antigen at low potential was developed by a multiple signal amplification strategy. Gold nanoparticles (AuNPs) served as carriers to load more secondary antibodies (Ab2) and horseradish peroxidase (HRP). The tip ultramicroelectrode was used to monitor the reduction current, and the 3-D images were obtained simultaneously. Under optimized conditions, the approach provided a linear response range from 1.0×l0−11 to 6.0×l0−11 M with a detection limit of 4.38×10–12 M. SECM is a versatile system that can be used not only for quantitative current analysis but also for topographic imaging of binding reaction. In addition, specific binding of antigen–antibody could also be continuously and successfully monitored by SECM. This immunoassay provides a sensitive approach for detecting tumor marker, and has potential application in clinical diagnostics.


► On the basis of anti-CD10 modified electrode, an electrochemical immunosensor for sensitive detection of CD10 antigen at low potential was developed by a multiple signal amplification strategy.
► Gold nanoparticles served as carriers to load more secondary antibodies (Ab2) and horseradish peroxidase (HRP).
► SECM is a versatile system that can be used not only for quantitative current analysis but also for topographic imaging of binding reaction; other specific binding of antigen–antibody could also be continuously and successfully monitored by SECM.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 38, Issue 1, October–December 2012, Pages 425–429
نویسندگان
, , ,