کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
867627 1470992 2011 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Rapid real-time electrical detection of proteins using single conducting polymer nanowire-based microfluidic aptasensor
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Rapid real-time electrical detection of proteins using single conducting polymer nanowire-based microfluidic aptasensor
چکیده انگلیسی

Single polypyrrole (PPy) nanowire-based microfluidic aptasensors were fabricated using a one-step electrochemical deposition method. The successful incorporation of the aptamers into the PPy nanowire was confirmed by fluorescence microscopy image. The microfluidic aptasensor showed responses to IgE protein solutions in the range from 0.01 nM to 100 nM, and demonstrated excellent specificity and sensitivity with faster response and rapid stabilization times (∼20 s). At the lowest examined IgE concentration of 0.01 nM, the microfluidic aptasensor still exhibited ∼0.32% change in the conductance. The functionality of this aptasensor was able to be regenerated using an acid treatment with no major change in sensitivity. In addition, the detection of cancer biomarker MUC1 was performed using another microfluidic aptasensor, which showed a very low detection limit of 2.66 nM MUC1 compared to commercially available MUC1 diagnosis assay (800 nM).

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 30, Issue 1, 15 December 2011, Pages 306–309
نویسندگان
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