کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
867678 909790 2011 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Glucose biosensor based on covalent immobilization of enzyme in sol–gel composite film combined with Prussian blue/carbon nanotubes hybrid
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Glucose biosensor based on covalent immobilization of enzyme in sol–gel composite film combined with Prussian blue/carbon nanotubes hybrid
چکیده انگلیسی

A novel electrochemical glucose biosensor was developed based on in situ covalent immobilization of glucose oxidase (GOx) by one-pot chitosan (CS)-incorporated sol–gel process in the presence of Prussian blue deposited multi-walled carbon nanotubes hybrids (PB/MWNTs) using 3-isocyanatopropyltriethoxysilane (ICPTES) as both a sol–gel precursor and a covalent coupling agent for GOx and CS. The electrode modified with the PB/MWNTs-GOx-CS-ICPTES sol–gel composite film showed good electrical conductivity and effective low-potential electron transfer mediation toward H2O2 reduction attributed to the incorporation of PB/MWNTs. The biosensor exhibited a linear response to glucose in the concentration range from 2.5 × 10−5 to 1.3 × 10−3 M with a correlation coefficient of 0.9998, a detection limit of 7.5 × 10−6 M, a low response time (10 s), good sensitivity and high anti-interference ability. Compared with the control biosensor based on the traditional tetraethoxysilane derived sol–gel composite film, the biosensor showed a similarly small apparent Michaelis–Menten constant of 3.67 mM but much higher electrochemical and biosensing stability.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 26, Issue 9, 15 May 2011, Pages 3973–3976
نویسندگان
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