کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
868473 | 909808 | 2010 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Aptamers based electrochemical biosensor for protein detection using carbon nanotubes platforms
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A label-free bioelectronic detection of aptamer–thrombin interaction based on electrochemical impedance spectroscopy (EIS) technique is reported. Multiwalled carbon nanotubes (MWCNTs) were used as modifiers of screen-printed carbon electrotransducers (SPCEs), showing improved characteristics compared to the bare SPCEs. 5′amino linked aptamer sequence was immobilized onto the modified SPCEs and then the binding of thrombin to aptamer sequence was monitored by EIS transduction of the resistance to charge transfer (Rct) in the presence of 5 mM [Fe(CN)6]3−/4−, obtaining a detection limit of 105 pM. This study represents an alternative electrochemical biosensor for the detection of proteins with interest for future applications.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 26, Issue 4, 15 December 2010, Pages 1715–1718
Journal: Biosensors and Bioelectronics - Volume 26, Issue 4, 15 December 2010, Pages 1715–1718
نویسندگان
Pinar Kara, Alfredo de la Escosura-Muñiz, Marisa Maltez-da Costa, Maria Guix, Mehmet Ozsoz, Arben Merkoçi,