کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
868567 | 909810 | 2010 | 6 صفحه PDF | دانلود رایگان |
In this study a biofuel cell anode is developed on the basis of multi-walled carbon nanotubes (MWCNTs). Recombinant pyrroloquinoline quinone (PQQ) dependent glucose dehydrogenase (GDH) is covalently coupled to a PQQ-layer which is adsorbed onto thiol-modified MWCNTs at a gold electrode. In the presence of glucose a catalytic current starts at a potential of −80 mV vs. Ag/AgCl, 1 M KCl. Under substrate saturation current densities of 170–200 μA/cm2 can be achieved. The operation is based on mediated electron transfer of the enzyme.This (PQQ)-GDH-MWCNT-electrode is combined with a MWCNT-modified electrode to which bilirubin oxidase (BOD) is covalently coupled. The resulting membrane-free biofuel cell has an open cell potential of 600 mV and can achieve a power density in the range of 23 μW/cm2.
Journal: Biosensors and Bioelectronics - Volume 26, Issue 2, 15 October 2010, Pages 530–535