کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
868710 | 909812 | 2009 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Fourier transform infrared immunosensors for model hapten molecules
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
We report experimental results concerning the detection of 2,4-dinitrophenol, under its free form or coupled to human serum albumin using Fourier transform infrared spectroscopy-based sensors. Competitive immunoreactions were carried out using several anti-dinitrophenol monoclonal antibodies. Comparison with enzyme-linked immunosorbent assays in competition is given for standard operating conditions. FTIR detection limits are comparable to those obtained by ELISA. The limits of detection are about 5–15 ng/mL for the coupled DNP. Using the LO-DNP61 antibody, a detection limit of ≅ 5 ng/mL was also estimated for the free DNP molecules but is much higher for the other antibodies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 24, Issue 8, 15 April 2009, Pages 2554–2558
Journal: Biosensors and Bioelectronics - Volume 24, Issue 8, 15 April 2009, Pages 2554–2558
نویسندگان
E. Gosselin, M. Gorez, M. Voué, O. Denis, J. Conti, N. Popovic, A. Van Cauwenberge, E. Noel, J. De Coninck,