کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
868710 909812 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Fourier transform infrared immunosensors for model hapten molecules
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Fourier transform infrared immunosensors for model hapten molecules
چکیده انگلیسی

We report experimental results concerning the detection of 2,4-dinitrophenol, under its free form or coupled to human serum albumin using Fourier transform infrared spectroscopy-based sensors. Competitive immunoreactions were carried out using several anti-dinitrophenol monoclonal antibodies. Comparison with enzyme-linked immunosorbent assays in competition is given for standard operating conditions. FTIR detection limits are comparable to those obtained by ELISA. The limits of detection are about 5–15 ng/mL for the coupled DNP. Using the LO-DNP61 antibody, a detection limit of ≅ 5 ng/mL was also estimated for the free DNP molecules but is much higher for the other antibodies.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 24, Issue 8, 15 April 2009, Pages 2554–2558
نویسندگان
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