کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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868798 | 909814 | 2010 | 5 صفحه PDF | دانلود رایگان |

A novel glucose biosensor based on immobilization of glucose oxidase in thin films of chitosan containing nanocomposites of graphene and gold nanoparticles (AuNPs) at a gold electrode was developed. The resulting graphene/AuNPs/chitosan composites film exhibited good electrocatalytical activity toward H2O2 and O2. The wide linear response to H2O2 ranging from 0.2 to 4.2 mM (R = 0.998) at −0.2 V, high sensitivity of 99.5 μA mM−1 cm−2 and good reproducibility were obtained. The good electrocatalytical activity might be attributed to the synergistic effect of graphene and AuNPs. With glucose oxidase (GOD) as a model, the graphene/AuNPs/GOD/chitosan composite-modified electrode was constructed through a simple casting method. The resulting biosensor exhibited good amperometric response to glucose with linear range from 2 to 10 mM (R = 0.999) at −0.2 V and from 2 to 14 mM (R = 0.999) at 0.5 V, good reproducibility and detection limit of 180 μM. Glucose concentration in human blood was studied preliminarily. From 2.5 to 7.5 mM, the cathodic peak currents of the biosensor decrease linearly with increasing the glucose concentrations. The graphene/AuNPs/GOD/chitosan composites film shows prominent electrochemical response to glucose, which makes a promising application for electrochemical detection of glucose.
Journal: Biosensors and Bioelectronics - Volume 25, Issue 5, 15 January 2010, Pages 1070–1074