Fabrication of a novel layer-by-layer film based glucose biosensor with compact arrangement of multi-components and glucose oxidase

Layer-by-layer (LbL) film based glucose biosensor was fabricated with alternative layers of a nanocomposite (comprising of multiwalled carbon nanotubes (MWNTs), Au nanoparticles (Au NPs) and thiol functionalized polyaniline (PANI(SH)) and glucose oxidase (GOx). The successful formation of multilayers was confirmed by UV–visible spectroscopy. The components in the nanocomposite provide adequate electron transfer path between GOx and the electrode. A high value for the rate constant of electron transfer process (27.84 s−1) was observed at {GOx/Au-(SH)PANI-g-MWNT}n/ITO electrode. The {GOx/Au-(SH)PANI-g-MWNT}n biosensor exhibited high sensitivity (3.97 μA/mM) for the detection of glucose over a concentration range of 1–9 mM with a low detection limit of 0.06 μM.