کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
869306 | 909827 | 2008 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Amperometric immunosensor for diagnosis of BLV infection
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Amperometric immunosensor for diagnosis of BLV infection Amperometric immunosensor for diagnosis of BLV infection](/preview/png/869306.png)
چکیده انگلیسی
A new amperometric immunosensor for detection of antibodies against bovine leukemia protein (gp51) was designed. The detection of antibody–antigen complex formation was based on application of secondary antibodies labeled with horseradish peroxidase (HRP). Ferrocenecarboxylic acid (FCA) and N,N,N′,N′-tetramethylbenzidine (TMB) were selected as suitable mediators for this immunosensor. Optimal conditions for amperometric detection were found. Sensitivity of created system was compared with the results of enzyme-linked immunosorbent assay (ELISA) and agar gel immunodiffusion (AGID) reaction, and was sufficient for detection of usual anti-gp51 antibody concentration present in the blood serum of BLV-infected cattle.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 23, Issue 10, 15 May 2008, Pages 1547–1554
Journal: Biosensors and Bioelectronics - Volume 23, Issue 10, 15 May 2008, Pages 1547–1554
نویسندگان
Bogumila Kurtinaitiene, Dovile Ambrozaite, Valdas Laurinavicius, Almira Ramanaviciene, Arunas Ramanavicius,