کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
869326 909828 2010 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Sensitive enzyme-biosensor based on screen-printed electrodes for Ochratoxin A
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Sensitive enzyme-biosensor based on screen-printed electrodes for Ochratoxin A
چکیده انگلیسی

Horseradish peroxidase has been successfully immobilized in a polypyrrole matrix onto disposable screen-printed carbon electrodes for the selective detection of Ochratoxin A. The chronoamperometric determination of this mycotoxin has been optimized by experimental design methodology, which implied the join evaluation of pH of the buffer solution, applied potential and concentration of H2O2. The slopes of the calibration curves built under the optimum conditions of the experimental variables have been used to estimate the reproducibility and the repeatability of the developed biosensor for Ochratoxin A. Relative standard deviation values of 1.9% (n = 5 and α = 0.05) and 7.1% (n = 4 and α = 0.05) were obtained for reproducibility and repeatability, respectively. The capability of detection for this method was 0.1 ng mL−1 (α = 0.05 and β < 0.05). The viability of the developed biosensor in the determination of Ochratoxin A in spiked beer and in roasted coffee has been shown, yielding average recoveries of 103% and 99%, in that order, with relative standard deviation values less than 5% (n = 3 and α = 0.05) in both cases.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 25, Issue 6, 15 February 2010, Pages 1333–1337
نویسندگان
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