کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
870254 909857 2005 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Biosensors based on acrylic microgels : A comparative study of immobilized glucose oxidase and tyrosinase
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Biosensors based on acrylic microgels : A comparative study of immobilized glucose oxidase and tyrosinase
چکیده انگلیسی

Acrylic microgels are proposed as enzyme immobilizing support in amperometric biosensors. Two enzymes, glucose oxidase and tyrosinase, were entrapped in this matrix and their behaviour is compared. The optimum cross-linking of the polymeric matrix required to retain the enzyme, and to allow the diffusion of the substrate is different for each enzyme, 3.2% for glucose oxidase and 4.5% for tyrosinase.The effect of pH and temperature on the biosensor responses has been studied by experimental design methodology and predictions have been compared with independently performed experimental measurements. A quadratic effect of the variables studied (pH and T) on the biosensor response and the small or null interaction between them was confirmed. The pH results obtained with both methods are coincident revealing an reversible effect on the enzyme. However, the temperature optimum value obtained by experimental design was 10 °C lower as a result of an activity decay due to irreversible thermal denaturation of both enzymes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 20, Issue 11, 15 May 2005, Pages 2268–2275
نویسندگان
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