کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
870539 909873 2006 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of a disposable pyruvate biosensor to determine pungency in onions (Allium cepa L.)
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Development of a disposable pyruvate biosensor to determine pungency in onions (Allium cepa L.)
چکیده انگلیسی

A disposable prototype pyruvate biosensor was constructed using pyruvate oxidase immobilised on mediated meldolas blue electrodes to determine pungency in onions (Allium cepa L.). The optimum operating potential was +150 mV (versus Ag/AgCl). A strong correlation between the biosensor response and untreated onion juice of known pyruvate concentration 2–12 μmol/g fresh weight (FW) was demonstrated. The biosensor was able to differentiate between low and high pungency onions. The detection limit using 1 unit of pyruvate oxidase was 1–2 μmol/g FW. Optimum concentrations of co-factors TPP, FAD and MgSO4 comprising the enzyme cocktail were determined as being 0.04, 0.1 and 30 mM, respectively.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 21, Issue 11, 15 May 2006, Pages 2176–2179
نویسندگان
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