A novel method to augment extraction of mangiferin by application of microwave on three phase partitioning

• Report novel technique for mangiferin extraction from Mangifera indica leaves.
• MTTP gives higher yield as compared with other conventional and novel techniques.
• It gives simultaneous extraction and purification of biomolecules in a short time.
• It can be used as a basis for separation of other molecules from natural source.

This work reports a novel approach where three phase partitioning (TPP) was combined with microwave for extraction of mangiferin from leaves of Mangifera indica. Soxhlet extraction was used as reference method, which yielded 57 mg/g in 5 h. Under optimal conditions such as microwave irradiation time 5 min, ammonium sulphate concentration 40% w/v, power 272 W, solute to solvent ratio 1:20, slurry to t-butanol ratio 1:1, soaking time 5 min and duty cycle 50%, the mangiferin yield obtained was 54 mg/g by microwave assisted three phase partitioning extraction (MTPP). Thus extraction method developed resulted into higher extraction yield in a shorter span, thereby making it an interesting alternative prior to down-stream processing.