کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8744583 | 1592813 | 2018 | 42 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Bioethanol production by a xylan fermenting thermophilic isolate Clostridium strain DBT-IOC-DC21
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موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
میکروب شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
To overcome the challenges associated with combined bioprocessing of lignocellulosic biomass to biofuel, finding good organisms is essential. An ethanol producing bacteria DBT-IOC-DC21 was isolated from a compost site via preliminary enrichment culture on a pure hemicellulosic substrate and identified as a Clostridium strain by 16S rRNA analysis. This strain presented broad substrate spectrum with ethanol, acetate, lactate, and hydrogen as the primary metabolic end products. The optimum conditions for ethanol production were found to be an initial pH of 7.0, a temperature of 70â¯Â°C and an L-G ratio of 0.67. Strain presented preferential hemicellulose fermentation when compared to various substrates and maximum ethanol concentration of 26.61â¯mM and 43.63â¯mM was produced from xylan and xylose, respectively. During the fermentation of varying concentration of xylan, a substantial amount of ethanol ranging from 25.27â¯mM to 67.29â¯mM was produced. An increased ethanol concentration of 40.22â¯mM was produced from a mixture of cellulose and xylan, with a significant effect observed on metabolic flux distribution. The optimum conditions were used to produce ethanol from 28â¯gâ¯Lâ1 rice straw biomass (RSB) (equivalent to 5.7â¯gâ¯Lâ1 of the xylose equivalents) in which 19.48â¯mM ethanol production was achieved. Thus, Clostridium strain DBT-IOC-DC21 has the potential to perform direct microbial conversion of untreated RSB to ethanol at a yield comparative to xylan fermentation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Anaerobe - Volume 51, June 2018, Pages 89-98
Journal: Anaerobe - Volume 51, June 2018, Pages 89-98
نویسندگان
Nisha Singh, Munish Puri, Deepak K. Tuli, Ravi P. Gupta, Colin J. Barrow, Anshu S. Mathur,