کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8746959 | 1593416 | 2018 | 18 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Development of recombinant goatpox virus expressing Echinococcus granulosus EG95 vaccine antigen
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Development of recombinant goatpox virus expressing Echinococcus granulosus EG95 vaccine antigen Development of recombinant goatpox virus expressing Echinococcus granulosus EG95 vaccine antigen](/preview/png/8746959.png)
چکیده انگلیسی
Goatpox disease and cystic hydatidosis may be simultaneously endemic in a given area. Their pathogens are goatpox virus (GPV) and Echinococcus granulosus (E. granulosus), respectively. Both E. granulosus EG95 subunit vaccine and live-attenuated GPV AV41 vaccine have been widely used for prevention of both diseases in China. However, it has been rarely reported that a bivalent vaccine is developed to prevent both diseases. The GPV is an ideal vector for developing recombinant multivalent vaccines to deliver immunogenic proteins in animals. In this study, we constructed an EG95 antigen-expressing recombinant GPV by the thymidine kinase gene-based homologous recombination in vitro. The recombinant GPV was purified and then proven to be able to express the EG95 antigen in vitro by Western blot and indirect immunoinfluscent assay, therefore expecting its potential as a bivalent vaccine candidate against both diseases in a future animal experiment.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 261, November 2018, Pages 28-33
Journal: Journal of Virological Methods - Volume 261, November 2018, Pages 28-33
نویسندگان
Fuxiao Liu, Xiaoxu Fan, Lin Li, Weijie Ren, Xiuju Han, Xiaodong Wu, Zhiliang Wang,