کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8749226 | 1593666 | 2018 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Isolation and characterization of Metarhizium anisopliae TK29 and its mycoinsecticide effects against subterranean termite Coptotermes formosanus
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
میکروب شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The entomopathogenic fungus Metarhizium anisopliae is widely used as biocontrol agent against many insect pests. In the present study, the potential isolate of M. anisopliae TK29 was isolated from the agricultural soils in Thekkady, India. The taxonomic identity of the isolate was confirmed based on its morphology and 18S rDNA gene sequence homology. Phylogenetic analysis confirmed that the isolated strains were related to the same species. A potential isolate (TK29) was optimized for mass cultivation and conidial spore production was enhanced using three different raw substrates (Rice, Maize, black gram) by solid-state fermentation. The results showed higher conidial spore yield from rice (2.6â¯Â±â¯0.32%) compared to black gram (2.1â¯Â±â¯0.28%) and maize (1.9â¯Â±â¯0.23%) substrates. Dry green conidia were applied against Formosan subterranean termite, Coptotermes formosanus at three different concentrations (1â¯Ãâ¯106, 1â¯Ãâ¯107, and 1â¯Ãâ¯108 conidia/mlâ1). The highest mortality rate was obtained from 1â¯Ãâ¯108 conidia/mlâ1â¯at 120â¯h post-treatment. Our study indicated that M. anisopliae TK29 had desirable attributes for the development of a mycoinsecticide against C. formosanus.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbial Pathogenesis - Volume 123, October 2018, Pages 52-59
Journal: Microbial Pathogenesis - Volume 123, October 2018, Pages 52-59
نویسندگان
Ravindran Keppanan, Sivaramakrishnan Sivaperumal, Luis Carlos Ramos Aguila, Mubasher Hussain, Bamisope Steve Bamisile, Chandra Kanta Dash, Liande Wang,