کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8749864 1593675 2018 32 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Integrated analysis of miRNA and mRNA expression profiling in bovine endometrial cells in response to lipopolysaccharide-stimulation
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروب شناسی
پیش نمایش صفحه اول مقاله
Integrated analysis of miRNA and mRNA expression profiling in bovine endometrial cells in response to lipopolysaccharide-stimulation
چکیده انگلیسی
MicroRNAs (miRNA) play an important role in regulating gene expression, making them important resources for exploring molecular mechanisms. Molecular mechanisms involved in the inflammatory responses of bovine endometrial cells induced by lipopolysaccharide (LPS) have not been widely studied. In the present study, miRNA and mRNA expression profiling of bovine endometrial cells treated with 1 μg/mL LPS for 24 h were evaluated by RNA-Seq (RNA-sequencing). The results showed that LPS induced 20 (11 up- and 9 down-regulated) differentially-expressed miRNAs and 108 (90 up- and 18 down-regulated) differentially-expressed mRNAs of bovine endometrial cells. The results for 5 mRNAs and 4 miRNAs were evaluated by quantitative real-time PCR (qRT-PCR) to validate the reliability of the RNA-seq data. Integrating analysis of the miRNA and mRNA expression profiles revealed 116 miRNA-target gene pairs. GO and KEGG pathway analysis of differentially expressed miRNAs and target genes predicted the likely roles of differentially expressed miRNAs in inflammatory responses in bovine endometrial cells induced by LPS. The reliability of the integrating analysis of the miRNA and mRNA data were validated by measuring the expression of three miRNA-target gene pairs by qRT-PCR. Our results improve the understanding of the role of miRNA involvement in inflammatory response of bovine endometrial cells induced by LPS.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbial Pathogenesis - Volume 114, January 2018, Pages 129-138
نویسندگان
, , , , ,