کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8765496 | 1597721 | 2018 | 27 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Phototoxic effect of aluminium-chlorine and aluminium-hydroxide phthalocyanines on Leishmania (l.) amazonensis
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کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
پزشکی و دندانپزشکی (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Phototoxic effect of aluminium-chlorine and aluminium-hydroxide phthalocyanines on Leishmania (l.) amazonensis Phototoxic effect of aluminium-chlorine and aluminium-hydroxide phthalocyanines on Leishmania (l.) amazonensis](/preview/png/8765496.png)
چکیده انگلیسی
This study investigated the activity of photosensitive phthalocyanines on promastigotes and amastigotes of Leishmania (L.) amazonensis. Aluminum phthalocyanine chloride (AlPcCl), Aluminum phthalocyanine hydroxide (AlPcOH) and zinc phthalocyanine (PcZn) were tested in the presence (matte red LED, potency of 2.5-2.3â¯Î¼W for 30â¯min) and absence of light against L. amazonensis promastigotes and the parasite viability was evaluated after 24, 48 and 72â¯h. The amastigote forms were treated with AlPcCl and AlPcOH, following the same lighting protocols described for the promastigote forms, being evaluated after 24â¯h. Cytotoxicity to human erythrocytes and peritoneal macrophages was also evaluated. The results showed that AlPcCl and AlPcOH in the presence of light have antileishmania activity, with leishmanistatic effects on promastigotes and amastigotes of L. amazonensis, without causing cytotoxicity to peritoneal macrophages and erythrocytes. The concentrations that inhibited 50% of the promastigote forms after 24â¯h of light exposure were 0.21â¯Â±â¯0.08â¯Î¼M for AlPcCl and 0.23â¯Â±â¯0.06â¯Î¼M for AlPcOH. In 48â¯h and 72â¯h after the treatment, the IC50 of AlPcCl was 0.13â¯Â±â¯0.02 and 0.12â¯Â±â¯0.03â¯Î¼M and for AlPcOH was 0.14â¯Â±â¯0.01â¯Î¼M and 0.11â¯Â±â¯0.01â¯Î¼M, respectively. PcZn showed no activity on promastigotes of L. amazonensis. This study showed a substantial photodynamic activity of the phthalocyanines AlPcCl and AlPcOH against intracellular amastigotes forms of L. amazonensis after irradiation, presenting IC50 values of 0.62â¯Â±â¯0.06â¯Î¼M and 0.92â¯Â±â¯0.12â¯Î¼M, respectively. These results support the possibility of using photodynamic therapy for the treatment of cutaneous leishmaniasis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Photodiagnosis and Photodynamic Therapy - Volume 21, March 2018, Pages 239-245
Journal: Photodiagnosis and Photodynamic Therapy - Volume 21, March 2018, Pages 239-245
نویسندگان
V. Nesi-Reis, T.R. Navasconi, D.S.S.L. Lera-Nosone, E.L. Oliveira, P.M. Barbosa, W. Caetano, T.G.V. Silveira, S.M.A. Aristides, N. Hioka, M.V.C. Lonardoni,