کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8839106 | 1613314 | 2016 | 22 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A standardized and automated method of perineuronal net analysis using Wisteria floribunda agglutinin staining intensity
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کلمات کلیدی
PNNCaudate/putamenWFAprelimbicECMROICPUPFCstandard deviation - انحراف معیارAutomated image analysis - تجزیه و تحلیل تصویر اتوماتیکPerineuronal net - خالص Perineuronalstandard error of the mean - خطای استاندارد میانگینnan - درprefrontal cortex - قشر prefrontalorbitofrontal cortex - قشر اوربیتوفرنتالExtracellular matrix - ماتریکس خارج سلولیSEM - مدل معادلات ساختاری / میکروسکوپ الکترونی روبشیHIP - مفصل ران یا مفصل هیپregion of interest - منطقه مورد نظرdorsal hippocampus - هیپوکامپ پشتیArbitrary Unit - واحد خودآموزWisteria floribunda agglutinin - ویستریا فلوریبند آگلوتیینین
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب سلولی و مولکولی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Perineuronal nets (PNNs) are aggregations of extracellular matrix molecules that are critical for plasticity. Their altered development or changes during adulthood appear to contribute to a wide range of diseases/disorders of the brain. An increasing number of studies examining the contribution of PNN to various behaviors and types of plasticity have analyzed the fluorescence intensity of Wisteria floribunda agglutinin (WFA) as an indirect measure of the maturity of PNNs, with brighter WFA staining corresponding to a more mature PNN and dim WFA staining corresponding to an immature PNN. However, a clearly-defined and unified method for assessing the intensity of PNNs is critical to allow us to make comparisons across studies and to advance our understanding of how PNN plasticity contributes to normal brain function and brain disease states. Here we examined methods of PNN intensity quantification and demonstrate that creating a region of interest around each PNN and subtracting appropriate background is a viable method for PNN intensity quantification that can be automated. This method produces less variability and bias across experiments compared to other published analyses, and this method increases reproducibility and reliability of PNN intensity measures, which is critical for comparisons across studies in this emerging field.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: IBRO Reports - Volume 1, December 2016, Pages 54-60
Journal: IBRO Reports - Volume 1, December 2016, Pages 54-60
نویسندگان
Megan L. Slaker, John H. Harkness, Barbara A. Sorg,