کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8839790 | 1613755 | 2018 | 33 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Neuroprotective effect of paeoniflorin on okadaic acid-induced tau hyperphosphorylation via calpain/Akt/GSK-3β pathway in SH-SY5Y cells
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کلمات کلیدی
NFTsμ-CalpainRAPApaeoniflorincalcium/calmodulin protein kinase II3-MAMAP2LiClCaMKII3-methyladenine - 3-متیل آدنینAutophagy - اتوفاژیOkadaic acid - اسید اوکادائیکAlzheimer’s disease - بیماری آلزایمرTau - خود راRapamycin - راپامایسینCAST - قالبlithium chloride - لیتیم کلریدneurofibrillary tangles - مگس های نوروفیبریلیالmicrotubule-associated protein-2 - پروتئین مرتبط با میکروتوبول-2Chloroquine - کلروکین
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Abnormal phosphorylation of tau, one of the most common symptoms of dementia, has become increasingly important in the study of the etiology and development of Alzheimer's disease. Paeoniflorin, the main bioactive component of herbaceous peony, is a monoterpene glycoside, which has been reported to exert beneficial effects on neurodegenerative disease. However, the effect of paeoniflorin on tauopathies remains ambiguous. SH-SY5Y cells were treated with okadaic acid (OA) for 8â¯h to induce tau phosphorylation and no cell death was observed. Optical microscopy results showed that paeoniflorin ameliorated okadaic acid induced morphological changes, including cell swelling and synapsis shortening. Western blotting data illustrated that paeoniflorin reversed okadaic acid induced tau hyperphosphorylation, which was enhanced by inhibiting the activities of calpain, Akt and GSK-3β. Transmission electron microscopy results showed that paeoniflorin alone can reduce the number of autophagosomes and stabilize the microtubule structure. In addition, calpastain and paeoniflorin enhance the effect of paeoniflorin on stabilizing microtubules. In addition, calpastain markedly enhanced the effect of paeoniflorin on reversing okadaic acid-lowered fluorescence intensity of both MAP-2 and β III-tubulin, two microtubule-associated proteins. This study shows that paeoniflorin protected SH-SY5Y cells against okadaic acid assault by interfering with the calpain/Akt/GSK-3β-related pathways, in which autophagy might be involved. Besides, paeoniflorin is found to relieve the stress response of the microtubule structure system caused by okadaic acid treatment. The results presented in this study suggest that paeoniflorin potentially plays an important role in tauopathies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1690, 1 July 2018, Pages 1-11
Journal: Brain Research - Volume 1690, 1 July 2018, Pages 1-11
نویسندگان
Xiao-Hui Ma, Wen-Jun Duan, You-Sheng Mo, Jun-Li Chen, Shi Li, Wei Zhao, Lei Yang, Sui-Qing Mi, Xin-Liang Mao, Hong Wang, Qi Wang,