کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8842390 | 1615539 | 2018 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Transcriptomic analysis of chicken cochleae after gentamicin damage and the involvement of four signaling pathways (Notch, FGF, Wnt and BMP) in hair cell regeneration
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کلمات کلیدی
TGF-βAntibiotic toxicityFPKMFDR5-bromo-2-deoxyuridineFGFMAPK - MAPKRegeneration - باززاییBrdU - بروموداکسی اوریدینtransforming growth factor-β - تبدیل فاکتور رشد βSerial analysis of gene expression - تجزیه و تحلیل سریال بیان ژنSAGE - حکیمDEG - شماfibroblast growth factor - فاکتور رشد فیبروبلاستTranscriptomic - متن ترانهBMP - مدیریت فرایند کسب و کارSignaling pathway - مسیر سیگنالینگfalse discovery rate - میزان کشف کاذبParvalbumin - پاروالبومینBone morphogenetic protein - پروتئین مورفوژنیک استخوانmitogen-activated protein kinase - پروتئین کیناز فعال با mitogenDifferentially expressed genes - ژن های متفاوت بیان شده است
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
سیستم های حسی
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چکیده انگلیسی
Unlike mammalian hair cells, which are essentially unable to regenerate after damage, avian hair cells have a robust capacity for regeneration. The prerequisite for understanding the above difference is knowing the genetic programming of avian hair cell regeneration. Although the major processes have been known, the precise molecular signaling that induces regeneration remains unclear. To address this issue, we performed a high-throughput transcriptomic analysis of gene expression during hair cell regeneration in the chick cochlea after antibiotic injury in vivo. A total of 16,588 genes were found to be expressed in the cochlea, of which about 1000 genes were differentially expressed among the four groups studied, i.e., 2 days (d) or 3â¯d post-treatment with gentamicin or physiological saline. The differentially expressed genes were distributed across approximately one hundred signaling pathways, including the Notch, MAPK (FGF), Wnt and TGF-β (BMP) pathways that have been shown to play important roles in embryonic development. Some differentially expressed genes (2-3 in each pathway) were further verified by qRT-PCR. After blocking Notch, FGF or BMP signaling, the number of regenerating hair cells and mitotic supporting cells increased. However, the opposite effect was observed after suppressing the Wnt pathway or enhancing BMP signaling. To our knowledge, the present study provided a relatively complete dataset of candidate genes and signaling pathways most likely involved in hair cell regeneration and should be a useful start in deciphering the genetic circuitry for inducing hair cell regeneration in the chick cochlea.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Hearing Research - Volume 361, April 2018, Pages 66-79
Journal: Hearing Research - Volume 361, April 2018, Pages 66-79
نویسندگان
Lingling Jiang, Jincao Xu, Ran Jin, Huanju Bai, Meiguang Zhang, Siyuan Yang, Xuebo Zhang, Xinwen Zhang, Zhongming Han, Shaoju Zeng,