کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8843564 | 1616229 | 2018 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Rationalizing and advancing the 3-MPBA SERS sandwich assay for rapid detection of bacteria in environmental and food matrices
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کلمات کلیدی
APCThe Centers for Disease Control and PreventionGASPVBNCTSBEPACDCSERSEnvironmental Protection Agency - آژانس حفاظت از محیط زیستEnzyme-linked immunosorbent assay - تست الیزاELISA - تست الیزاBacteria detection - تشخیص باکتریAerobic Plate Count - تعداد صفحات هوازیTryptic soy broth - سوپ سویا TrypticSurface-enhanced Raman spectroscopy - طیف سنجی رامان با سطح افزایش یافتهViable but nonculturable - قابل اعتماد اما غیرقابل پذیرش استFood matrices - ماتریسهای غذاییpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمراز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Bacterial foodborne illness continues to be a pressing issue in our food supply. Rapid detection methods are needed for perishable foods due to their short shelf lives and significant contribution to foodborne illness. Previously, a sensitive and reliable surface-enhanced Raman spectroscopy (SERS) sandwich assay based on 3-mercaptophenylboronic acid (3-MBPA) as a capturer and indicator molecule was developed for rapid bacteria detection. In this study, we explored the advantages and constraints of this assay over the conventional aerobic plate count (APC) method and further developed methods for detection in real environmental and food matrices. The SERS sandwich assay was able to detect environmental bacteria in pond water and on spinach leaves at higher levels than the APC method. In addition, the SERS assay appeared to have higher sensitivity to quantify bacteria in the stationary phase. On the other hand, the APC method was more sensitive to cell viability. Finally, a method to detect bacteria in a challenging high-sugar juice matrix was developed to enhance bacteria capture. This study advanced the SERS technique for real applications in environment and food matrices.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Microbiology - Volume 72, June 2018, Pages 89-97
Journal: Food Microbiology - Volume 72, June 2018, Pages 89-97
نویسندگان
Brooke Pearson, Alexander Mills, Madeline Tucker, Siyue Gao, Lynne McLandsborough, Lili He,