کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8850888 | 1618765 | 2018 | 33 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Chitin synthesis inhibitors promote liver cancer cell metastasis via interfering with hypoxia-inducible factor 1α
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کلمات کلیدی
hypoxia-inducible factor 1α (HIF-1α)ERβERαHREsHIF-1αECMTIMP-2PPARγMMP-2CD-FBSFN1HepG2CSISFBSDMEMAHRChitin synthesis inhibitorsPBSRLA1,10-Phenanthroline - 1،10-PhenanthrolinecDNA - cDNADMSO - DMSOE-cad - E-CADE-cadherin - E-CadherinDulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده DulbeccoEMT - تکنسین فوریتهای پزشکیDimethyl sulfoxide - دیمتیل سولفواکسیدfetal bovine serum - سرم جنین گاوhypoxia-inducible factor 1α - عامل القایی هیپوکسی 1αFibronectin - فیبرونکتینExtracellular matrix - ماتریکس خارج سلولیMatrix metalloproteinase-2 - ماتریکس متالوپروتئیناز-2Phosphate-buffered saline - محلول نمک فسفات با خاصیت بافریTissue inhibitor of metalloproteinases-2 - مهار کننده های متالوپروتئیناز-2epithelial-to-mesenchymal transition - گذار اپیتلیال به مزانشیمالaryl hydrocarbon receptor - گیرنده آرویل هیدروکربنAndrogen Receptor - گیرنده آندروژنیperoxisome proliferator-activated receptor γ - گیرنده پروتئین کننده پروکسیوم فعال γ
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
شیمی زیست محیطی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Chitin synthesis inhibitors (CSIs), as alternatives to conventional insecticides, have been in worldwide demand in recent years. However, little attention has been paid to the potential ecological safety and health risks of CSIs, especially their abilities to interfere with nonsexual hormone receptors such as hypoxia-inducible factor 1α (HIF-1α). In this work, we conducted a systematic study regarding the influence of CSIs on HIF-1α-related liver cancer cell metastasis. The dual-luciferase reporter gene assay revealed that two of fourteen CSIs exhibited dose-response HIF-1α agonistic activities at noncytotoxic concentrations with relative luciferase activity (RLA) values of 25.6% for diflubenzuron (DFB) and 20.9% for triflumuron (TFM). Following this result, in vitro bioassays demonstrated that both DFB and TFM stimulated HepG2 cell migration and invasion. This action was associated with the varied expression levels of genes involved in epithelial-to-mesenchymal transition (EMT) activation and extracellular matrix (ECM) degradation, such as the upregulation of fibronectin (FN1) and matrix metalloproteinase-2 (MMP-2) and the suppression of E-cadherin (E-cad) and tissue inhibitor of metalloproteinases-2 (TIMP-2). Moreover, changes in these EMT and ECM phenotype markers were dramatically blocked by a HIF-1α inhibitor (KC7F2), which further verified the involvement of HIF-1α in CSI-induced HepG2 cell metastasis. For the first time, our findings reveal that CSIs play crucial roles in promoting the metastasis of human liver cancer cells and that HIF-1α is potentially responsible for these changes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemosphere - Volume 206, September 2018, Pages 231-237
Journal: Chemosphere - Volume 206, September 2018, Pages 231-237
نویسندگان
Xia Ning, Yue Wang, Wei Yan, Guangke Li, Nan Sang,