The in vivo action of chronic bisphenol F showing potential immune disturbance in juvenile common carp (Cyprinus carpio)

Bisphenol F (BPF) has been increasingly introduced into industrial applications as a replacement for bisphenol A (BPA), and has emerged as a ubiquitous environmental contaminant worldwide. Few studies have assessed the in vivo toxicities of BPF, particularly long-term exposure toxicities. In the present study, we examined whether long-term BPF exposure in vivo would evoke oxidative stress in the immune system of juvenile common carp. The results suggested that BPF exposure increased ROS content, oxidative stress indices, complement component 3, and immunoglobulin M contents, as well as the expression of inflammatory cytokine genes. Moreover, higher levels of nf-κb p65 gene expression were correlated with the induced ROS content and NF-κB pathway-associated genes, a strong indication that the mode of action of BPF is related to the NF-κB signaling pathway. We also provide evidence that the effects of BPF are comparable to those of BPA with regards to regulation of the immune response in teleosts, and therefore suggest that such chemical analogs should be thoroughly evaluated for their potential toxicity before they can be considered as “safer” replacements.