کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8851483 | 1618770 | 2018 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
In vivo visual reporter system for estrogenic contaminant exposure using transgenic see-through Japanese medaka Oryzias latipes
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
شیمی زیست محیطی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: In vivo visual reporter system for estrogenic contaminant exposure using transgenic see-through Japanese medaka Oryzias latipes In vivo visual reporter system for estrogenic contaminant exposure using transgenic see-through Japanese medaka Oryzias latipes](/preview/png/8851483.png)
چکیده انگلیسی
Estrogenic contaminants are commonly detected in water bodies and can result in reproductive effects of aquatic organisms. This necessitates the development of time and cost effective tools to screen chemicals for estrogenic potential. Our objective was to develop a novel in vivo visual reporter system for rapid detection of estrogenic contaminants. We developed a Japanese medaka (Oryzias latipes) transgenic line (pOSP1-AcGFP), which expresses Aequorea coerulescens green florescence protein (AcGFP) under the control of ovarian structural protein 1 (osp1) promoter region. Osp1 is a gene with early female-specific expression that is highly responsive to hormonal exposure. Next, we conducted 24â¯h exposure of 30 dph pOSP1-AcGFP larvae to successfully detect estrogenic activity of contaminants. Our results support the hypothesis that molecular biomarkers are sensitive tools for early detection of the effects of estrogenic contaminants.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemosphere - Volume 201, June 2018, Pages 251-253
Journal: Chemosphere - Volume 201, June 2018, Pages 251-253
نویسندگان
Ahmed Abdel-moneim, Cecon T. Mahapatra, Maria S. Sepúlveda,