کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8852885 | 1618780 | 2018 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Comparison of in vitro and in vivo bioassays to measure thyroid hormone disrupting activity in water extracts
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
MMIDOCtransthyretinSPEInhibition concentrationTHBPPCPTRIACTBGTTRTPOSurface water - آبهای سطحیSolid-phase extraction - استخراج فاز جامدTetraiodothyroacetic acid - اسید تترادیوتررویکسونtriiodothyroacetic acid - اسید ترییدوتیروئیدTetrac - تتراسTriiodothyronine - تری یدوتیرونینThyroperoxidase - تیروپراکسیدازthyroxine - تیروکسینETU - جلوIn vivo - در داخل بدنIn vitro - در فضای بازEffect concentration - غلظت اثرWastewater - فاضلاب یا پسابMethimazole - متیمزولREF - مرجعThyroid hormone - هورمون تیروئیدPentachlorophenol - پنتا کلروفنلDissolved organic carbon - کربن آلی حل شدهthyroid binding globulin - گلوبولین اتصال دهنده تیروئیدThyroid receptor - گیرنده تیروئید
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
شیمی زیست محیطی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Environmental chemicals can induce thyroid disruption through a number of mechanisms including altered thyroid hormone biosynthesis and transport, as well as activation and inhibition of the thyroid receptor. In the current study six in vitro bioassays indicative of different mechanisms of thyroid disruption and one whole animal in vivo assay were applied to 9 model compounds and 4 different water samples (treated wastewater, surface water, drinking water and ultra-pure lab water; both unspiked and spiked with model compounds) to determine their ability to detect thyroid active compounds. Most assays correctly identified and quantified the model compounds as agonists or antagonists, with the reporter gene assays being the most sensitive. However, the reporter gene assays did not detect significant thyroid activity in any of the water samples, suggesting that activation or inhibition of the thyroid hormone receptor is not a relevant mode of action for thyroid endocrine disruptors in water. The thyroperoxidase (TPO) inhibition assay and transthyretin (TTR) displacement assay (FITC) detected activity in the surface water and treated wastewater samples, but more work is required to assess if this activity is a true measure of thyroid activity or matrix interference. The whole animal Xenopus Embryonic Thyroid Assay (XETA) detected some activity in the unspiked surface water and treated wastewater extracts, but not in unspiked drinking water, and appears to be a suitable assay to detect thyroid activity in environmental waters.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemosphere - Volume 191, January 2018, Pages 868-875
Journal: Chemosphere - Volume 191, January 2018, Pages 868-875
نویسندگان
Frederic D.L. Leusch, Natalie H. Aneck-Hahn, Jo-Anne E. Cavanagh, David Du Pasquier, Timo Hamers, Armelle Hebert, Peta A. Neale, Marco Scheurer, Steven O. Simmons, Merijn Schriks,