کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8883783 | 1625907 | 2018 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Short-term developmental effects and potential mechanisms of azoxystrobin in larval and adult zebrafish (Danio rerio)
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کلمات کلیدی
MDACATGPXRT-qPCROECDCyt CROS - ROSAzoxystrobin - آزوسیستروبینDevelopmental effects - اثرات توسعهMembrane degradation - تخریب غشاءMitochondrial swelling - تورم میتوکندریOrganization for Economic Co-operation and Development - سازمان همکاری و توسعه اقتصادیSOD - سدSuperoxide dismutase - سوکسوکس دیسموتازcytochrome c - سیتوکروم سیMembrane Rupture - غشاء شکستmalondialdehyde - مالون دی آلدهیدreal-time quantitative polymerase chain reaction - واکنش زنجیره ای پلیمراز کمی زمان واقعی استCatalase - کاتالازglutathione peroxidase - گلوتاتیون پراکسیدازZebrafish - گورخرماهیReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم آبزیان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Previous study indicated that azoxystrobin had high acute toxicity to zebrafish, and larval zebrafish were more sensitive to azoxystrobin than adult zebrafish. The objective of the present study was to investigate short-term developmental effects and potential mechanisms of azoxystrobin in larval and adult zebrafish. After zebrafish embryos and adults were exposed to 0.01, 0.05 and 0.20â¯mg/L azoxystrobin (equal to 25, 124 and 496â¯nM azoxystrobin, respectively) for 8â¯days, the lethal effect, physiological responses, liver histology, mitochondrial ultrastructure, and expression alteration of genes related to mitochondrial respiration, oxidative stress, cell apoptosis and innate immune response were determined. The results showed that there was no significant effect on larval and adult zebrafish after exposure to 0.01â¯mg/L azoxystrobin. However, increased ROS, MDA concentration and il1b in larval zebrafish, as well as increased il1b, il8 and cxcl-c1c in adult zebrafish were induced after exposure to 0.05â¯mg/L azoxystrobin. Reduced mitochondrial complex III activity and ATP concentration, increased SOD activity, ROS and MDA concentration, decreased cytb, as well as increased sod1, sod2, cat, il1b, il8 and cxcl-c1c were observed both in larval and adult zebrafish after exposure to 0.20â¯mg/L azoxystrobin; meanwhile, increased p53, bax, apaf1 and casp9, alteration of liver histology and mitochondrial ultrastructure in larval zebrafish, and alteration of mitochondrial ultrastructure in adult zebrafish were also induced. The results demonstrated that azoxytrobin induced short-term developmental effects on larval zebrafish and adult zebrafish, including mitochondrial dysfunction, oxidative stress, cell apoptosis and innate immune response. Statistical analysis indicated that azoxystrobin induced more negative effects on larval zebrafish, which might be the reason for the differences of developmental toxicity between larval and adult zebrafish caused by azoxystrobin. These results provided a new insight into potential mechanisms of azoxystrobin in larval zebrafish and adult zebrafish.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Aquatic Toxicology - Volume 198, May 2018, Pages 129-140
Journal: Aquatic Toxicology - Volume 198, May 2018, Pages 129-140
نویسندگان
Fangjie Cao, Peizhuo Wu, Lan Huang, Hui Li, Le Qian, Sen Pang, Lihong Qiu,