Digital PCR for analysis of peanut and soybean allergens in foods

- The efficient extraction method and optimal test portion were established.
- Digital PCR for allergens analysis is proposed.
- ELISA, qPCR and ddPCR were tested on market samples.
- Soybean was detected as hidden allergen in mill and bakery products.
- Digital PCR is suitable and reliable technology for peanut and soybean allergens analysis.