کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8888196 1628379 2018 27 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Inhibition effects of low concentrations of epigallocatechin gallate on the biofilm formation and hemolytic activity of Listeria monocytogenes
ترجمه فارسی عنوان
اثرات مهار کردن غلظت های پایین اپیگلاکاتچین گالات بر شکل گیری بیوفیلم و فعالیت همولیتیکی لیستریا
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
چکیده انگلیسی
Listeria monocytogenes (L. monocytogenes) is a well-known food-borne pathogen that causes systemic listeriosis. Its biofilm-forming ability is known to be important for its antimicrobial resistance and persistence. Epigallocatechin-gallate (EGCG) is the highest component of tea polyphenols in tea extracts and has broad-spectrum antimicrobial activities. In this study, the efficacys of EGCG to inhibit biofilm formation and hemolytic activity of L. monocytogenes were determined. EGCG at 20 μg/mL, 40 μg/mL (less than compound's minimum inhibitory concentration, MIC) and 200 μg/mL (1 MIC) were tested. Crystal violet staining showed that sub-MIC and MIC of EGCG could significantly reduce the biofilm formation by L. monocytogenes on polystyrene microtiter plates at three temperatures (15 °C, 30 °C and 37 °C). EGCG reduced the sessile cell numbers present in biofilm at those temperatures. EGCG also significantly inhibited hemolytic activity of L. monocytogenes as measured by sheep red blood cells. Real time PCR assay was used to investigate the relative gene expression of L. monocytogenes grown at 37 °C and revealed that EGCG down-regulated virulence genes (inlA and hly), SOS response genes (recA and yneA) and quorum sensing gene (agrA). These results suggested the feasibility of using EGCG in food industry to control L. monocytogenes biofilm formation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Control - Volume 85, March 2018, Pages 119-126
نویسندگان
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