کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8989561 | 1555478 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A novel genetic marker to differentiate feline herpesvirus type 1 field isolates
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A novel genetic marker to differentiate feline herpesvirus type 1 field isolates A novel genetic marker to differentiate feline herpesvirus type 1 field isolates](/preview/png/8989561.png)
چکیده انگلیسی
Five recent field isolates of feline herpesvirus type 1 (FHV-1) were compared by digestion with a restriction endonuclease, SalI or MluI. The SalI digestion showed a potentially useful difference in one isolate 00-035 that had an approximately 3.0Â kbp fragment instead of a 2.6Â kbp fragment in the other strains. After cloning the 3.0 and 2.6Â kbp fragments, the nucleotide sequences were analyzed. The result showed that the 3.0Â kbp fragment of 00-035 included a complete open reading frame of the herpes simplex virus 1 (HSV-1) homologue of the UL17 gene and a 5â²-part of UL16 gene and that only one nucleotide substitution was found in the 5â²-region of UL17 gene where the SalI site of the 2.6Â kbp fragment locates. Based on these nucleotide sequences, two PCR primers were designed to amplify the region around the SalI site in the UL17 gene and the PCR was carried out using 78 field isolates from various parts of Japan. The SalI digestion of the PCR products revealed an interesting profile in that the genotype without the SalI site in UL17 gene was dominant in Tottori and Yamagata prefectures (69% and 75%, respectively) but minor in the other regions of Japan (0-10%). These results suggest that the SalI digestion method described in the present study can be used as a genetic marker to differentiate some FHV-1 field isolates and this is the first report that showed different distributions of FHV-1 genotypes using the novel genetic marker.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Microbiology - Volume 106, Issues 3â4, 10 April 2005, Pages 195-200
Journal: Veterinary Microbiology - Volume 106, Issues 3â4, 10 April 2005, Pages 195-200
نویسندگان
Masataka Hamano, Ken Maeda, Kazushige Kai, Masami Mochizuki, Yukinobu Tohya, Hiroomi Akashi,