Rapid isolation of Brachyspira hyodysenteriae and Brachyspira pilosicoli from pigs

Spirochaetes from samples in S400, CVS and BJ, with and without pre-treatment, were obtained in pure cultures only after repeatedly transferring on plates of the same selective medium requiring 15-18 days according to the strain. BAM-SR used after the pre-treatment step showed a detection limit ranging from 3.5 × 102 to 6.7 × 107 cells/g faeces and was the only method able to support the growth of spirochaetes after 48 h.