کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8989711 | 1555476 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Insertion sequence profiling of UK Mycoplasmabovis field isolates
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The presence and distribution of insertion sequences ISMbov2 and ISMbov3 within Mycoplasma bovis were investigated. Analysis was carried out by Southern blotting using specific probes of 221Â bp and 185Â bp, to detect ISMbov2 and ISMbov3, respectively, amplified from the homologous sequences ISMmy1 and IS1634 within Mycoplasma mycoides subspecies mycoides small colony type. We present data obtained from 49 field isolates of M. bovis, originating from pneumonic lungs, collected within the United Kingdom between 1996 and 2002. Hybridisation profiles show considerable variation between strains. ISMbov2 sequences are present between 2 and 17 copies while there are between 3 and 14 copies of the IS1634 homologue ISMbov3. These data also provide support for previous analysis by random amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Microbiology - Volume 107, Issues 3â4, 20 May 2005, Pages 301-306
Journal: Veterinary Microbiology - Volume 107, Issues 3â4, 20 May 2005, Pages 301-306
نویسندگان
Katie Miles, Laura McAuliffe, Anja Persson, Roger D. Ayling, Robin A.J. Nicholas,