کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9000343 | 1116518 | 2005 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Differential immunoexpressions of cytoskeletons in renal epithelial and interstitial cells in rat and canine fibrotic kidneys, and in kidney-related cell lines under fibrogenic stimuli
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
ECMBMP-7CDDPHGFFGF-2FBSPDGF-BBTGF-β1PBSα-SMAUUOEGFBone morphogenic protein-7 - استخوان مورفوژن پروتئین -7standard deviation - انحراف معیارUnilateral ureteral obstruction - انسداد مجاری مدفوع یک طرفهα-smooth muscle actin - اکتین عضله آلفا صافTransforming growth factor-β1 - تبدیل فاکتور رشد β1EMT - تکنسین فوریتهای پزشکیminimum essential medium - حداقل حداقل مورد نیازfetal bovine serum - سرم جنین گاوcisplatin - سیس پلاتینepidermal growth factor - عامل رشد اپیدرمیHepatocyte growth factor - عامل رشد هپاتوسیتplatelet-derived growth factor-BB - عامل رشد پلاکتی-BBGrowth factor - فاکتور رشدPhosphate buffered saline - فسفات بافر شورfibroblast growth factor-2 - فیبروبلاست رشد فاکتور 2Extracellular matrix - ماتریکس خارج سلولیMEM - مامانHematoxylin and Eosin - هماتوکسیلین و ائوزینEpithelial-mesenchymal transition - گذار اپیتلیال-مزانشیمی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Myofibroblasts play an important role in chronic renal interstitial fibrosis. However, the origin and developmental mechanisms remain to be elucidated. The myofibroblasts may express various cytoskeletons during the development. Immunoexpressions of vimentin, desmin and α-smooth muscle actin (α-SMA) were analyzed using experimentally (cisplatin and unilateral ureteral obstruction) induced rat and spontaneous canine fibrotic kidneys or kidney-related cell lines incubated with transforming growth factor-β1 (TGF-β1), platelet-derived growth factor-BB (PDGF-BB) or their combination at various concentrations. In rat fibrotic kidneys, both renal epithelia and interstitial cells showed positive reactions to α-SMA and vimentin, supporting epithelial-mesenchymal transition (EMT) theory; however, renal epithelia did not react to desmin, though interstitial cells were reactive. Renal epithelia in canine fibrotic kidneys did not show a positive reaction to α-SMA, whereas interstitial cells reacted strongly to α-SMA; conversely, renal epithelia reacted strongly to desmin, but interstitial cells did not; vimentin expression was infrequently seen in renal epithelia and interstitial cells of canine kidneys. Exposure of TGF-β1 to porcine renal epithelial cells (LLC-PK1), rat renal interstitial cells (NRK-49F), and rat immature mesenchymal cells (MT-9) dose-dependently increased selectively α-SMA-positive cell numbers. Moreover, PDGF-BB exhibited an additive effect on TGF-β1-induced α-SMA expression in these cell lines when simultaneously added. α-SMA was the most plastic cytoskeleton under fibrogenic stimuli. This study shows that there are interspecies differences in cytoskeletal immunoexpressions of renal epithelia or interstitial cells between rat and canine fibrotic kidneys, and that the derivation of renal myofibroblasts may be heterogeneous, such as renal epithelia, interstitial cells or immature mesenchymal cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental and Toxicologic Pathology - Volume 57, Issue 2, 15 November 2005, Pages 135-147
Journal: Experimental and Toxicologic Pathology - Volume 57, Issue 2, 15 November 2005, Pages 135-147
نویسندگان
Jyoji Yamate, Masanori Kuribayashi, Mitsuru Kuwamura, Takao Kotani, Kikumi Ogihara,