کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9001416 | 1118516 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Phosphorylation of 9-β-d-arabinofuranosylguanine monophosphate by Drosophila melanogaster guanylate kinase
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کلمات کلیدی
AragdNKGUK9-β-d-arabinofuranosylguanineNucleoside analog - آنالوگ هسته ایAnti-cancer therapy - درمان ضد سرطانSuicide gene therapy - درمان ژن خودکشیDeoxyribonucleoside kinase - دز اکسید ریبونوکلئوزید کینازDrosophila melanogaster - مگس سرکه، مگس میوهPurine - پورینNucleoside kinase - کیناز نوکلئوزیدGuanylate kinase - گینیلات کیناز
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
داروشناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Phosphorylation of 9-β-d-arabinofuranosylguanine monophosphate by Drosophila melanogaster guanylate kinase Phosphorylation of 9-β-d-arabinofuranosylguanine monophosphate by Drosophila melanogaster guanylate kinase](/preview/png/9001416.png)
چکیده انگلیسی
Nucleoside monophosphate kinases have an important role in the synthesis of nucleotides that are required for cellular metabolism. These enzymes are also important for the phosphorylation of nucleoside- and nucleotide analogs used in cancer and anti-viral therapy. We report the cDNA cloning and characterization of a 23 kDa guanylate kinase from Drosophila melanogaster (Dm-GUK). The predicted amino acid sequence was 58% identical to the human guanylate kinase and the enzyme was shown to phosphorylate GMP and dGMP with ATP as phosphate donor. The monophosphates of the deoxyguanosine analogs 2â²,2â²-difluorodeoxyguanosine (dFdG) and 9-β-d-arabinofuranosylguanine (araG) were also shown to be phosphorylated by the enzyme. We used the enzyme to reconstitute the complete in vitro three-step phosphorylation pathway for the conversion of dGuo and araG to the corresponding triphosphates.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Pharmacology - Volume 70, Issue 7, 1 October 2005, Pages 987-992
Journal: Biochemical Pharmacology - Volume 70, Issue 7, 1 October 2005, Pages 987-992
نویسندگان
Magnus Johansson, Marjan Amiri, Anna Karlsson,