کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9002171 | 1118576 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Antimetastatic effect of prodigiosin through inhibition of tumor invasion
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کلمات کلیدی
ECMMmpsi.v.RhoAEC50i.p.MTT - MTTsodium dodecyl sulfate-polyacrylamide gel electrophoresis - الکتروفورز ژل دوده سولفات سدیم پلی آکریل آمیدSDS-PAGE - الکتروفورز ژل پلی آکریل آمیدInvasion - تهاجمIntraperitoneally - داخل صفاقیintravenously - داخل وریدیreverse transcription - رونویسی معکوسbovine serum - سرم گاوExtracellular matrix - ماتریکس خارج سلولیMetastasis - متاستاز Matrix metalloproteinases - متالوپروتئیناز ماتریکسProdigiosin - پرودیگیوزین
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
داروشناسی
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
Prodigiosin, a bacterial metabolite, was reported to have immunosuppressive and anticancer activities. In this study, we investigated novel functions of prodigiosin about anti-metastasis and anti-invasion. Prodigiosin dose-dependently inhibited 95-D cells' migration and invasion according to wound healing assay and the Transwell assay. The inhibitive effect could reach about 50% when cells were treated with 5 μM prodigiosin for 12 h. In animal experiment, intraperitoneal administration of 5 mg kgâ1 prodigiosin decreased the number of metastatic nodules by 53% and elevated the survival rate of mice about one-fold comparing with control group. Results of cell aggregation and adhesion assay showed that prodigiosin could promote cell aggregation and simultaneously inhibit cell from adhering to extracellular matrix (ECM). In addition, prodigiosin suppressed RhoA gene expression, hence, decreased protein level of RhoA in 95-D cells, according to RT-PCR assay and Western blot assay. Gel zymogram assay revealed that prodigiosin could suppress the activity of matrix metalloproteinase-2 (MMP-2). These results demonstrate that prodigiosin effectively inhibit tumor metastasis in vitro and in vivo. The action mechanisms of prodigiosin are associated with the promotion of cell aggregation and the inhibition of various steps in cell invasive process, which include the inhibition of cell adhesion and mobility in a RhoA-dependent way and the suppression of MMP-2 ability.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Pharmacology - Volume 69, Issue 3, 1 February 2005, Pages 407-414
Journal: Biochemical Pharmacology - Volume 69, Issue 3, 1 February 2005, Pages 407-414
نویسندگان
Jing Zhang, Yaling Shen, Jianwen Liu, Dongzhi Wei,