کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9002239 | 1118578 | 2005 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The novel targets for anti-angiogenesis of genistein on human cancer cells
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کلمات کلیدی
MmpsMT-MMPPAI-1uPAPDGFTIMP-2RT-PCRCOX-2TSP-1tPAThrombospondin-1 - ترومبوسپوندن-1CAM - ساخت به کمک کامپیوترBladder cancer - سرطان مثانهCyclooxygenase-2 - سیکلوکوکسیژناز2Tissue factor - عامل بافتAngiogenic factors - عوامل آنژیوژنیکChick chorioallantoic membrane - غشای کوریوالانتانیک جوجهVascular endothelial growth factor - فاکتور رشد اندوتلیال عروقیplatelet-derived growth factor - فاکتور رشد حاصل از پلاکتTissue-type plasminogen activator - فعال کننده بافت پلاسمینوژنGenistein - ماده جنستئینMatrix metalloproteinases - متالوپروتئیناز ماتریکسtissue inhibitor of metalloproteinase-2 - مهار کننده بافت متالوپروتئیناز-2Plasminogen activator inhibitor-1 - مهار کننده فعال کننده پلاسمینوژن-1Angiogenesis inhibitors - مهار کننده های آنژیوژنزreverse transcription-polymerase chain reaction - واکنش زنجیره ای رونویسی-پلیمراز معکوس
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
داروشناسی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Genistein has been reported to be a natural chemopreventive in several types of human cancer. In our prior study, soy isoflavones were shown to induce cell cycle arrest and apoptosis of bladder cancer cells in the range of human urine excretion. This study was designed to identify the novel molecular basis underlying anti-angiogenic activities of soy isoflavones. An immortalized E6 and five human bladder cancer cell lines were studied by immunoassay, flow cytometry, functional activity, reverse transcription-polymerase chain reaction, immunoblotting, and transwell co-culture in vitro. The efficacy of soy isoflavones on angiogenesis inhibition in vivo was examined by nude mice xenograft and chick chorioallantoic membrane bioassay. Factors analyzed included angiogenic factors, matrix-degrading enzymes, and angiogenesis inhibitors. Genistein was the most potent inhibitor of angiogenesis in vitro and in vivo among the isoflavone compounds tested. It may also account for most of the reduced microvessel density of xenografts observed and the suppressed endothelial migration by soy isoflavones. Genistein exhibited a dose-dependent inhibition of expression/excretion of vascular endothelial growth factor165, platelet-derived growth factor, tissue factor, urokinase plasminogen activator, and matrix metalloprotease-2 and 9, respectively. On the other hand, there was an up-regulation of angiogenesis inhibitors-plasminogen activator inhibitor-1, endostatin, angiostatin, and thrombospondin-1. In addition, a differential inhibitory effect between immortalized uroepithelial cells and most cancer cell lines was also observed. Altogether, we discovered that tissue factor, endostatin, and angiostatin are novel molecular targets of genistein. The current investigation provides further evidence in support of soy-based foods as natural dietary inhibitors of tumor angiogenesis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Pharmacology - Volume 69, Issue 2, 15 January 2005, Pages 307-318
Journal: Biochemical Pharmacology - Volume 69, Issue 2, 15 January 2005, Pages 307-318
نویسندگان
Shu-Jem Su, Trai-Ming Yeh, Woei-Jer Chuang, Chung-Liang Ho, Kee-Lung Chang, Hsiao-Ling Cheng, Hsiao-Sheng Liu, Hong-Lin Cheng, Pei-Yin Hsu, Nan-Haw Chow,