کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9002308 | 1118583 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Inducible nitric oxide synthase-dependent stimulation of PKGI and phosphorylation of VASP in human embryonic kidney cells
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کلمات کلیدی
IBMXcGMPiNOSH89NG-monomethyl-L-arginineODQl-NMA3-isobutyl-1-methylxanthine - 3-ایزوبوتیل-1-متیلکسانتینcAMP - cAMPDEA/NO - DEA / NODETA/NO - DETA / NOhuman embryonic kidney 293 cells - سلول های انسانی جنینی انسان 293HEK cells - سلولهای HEKinducible nitric oxide synthase - سنتاز اکسید نیتریک القاییNitric oxide - نیتریک اکسیدguanylyl cyclase - گویینیل سیکلاس
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
داروشناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Inducible nitric oxide synthase (iNOS) production of nitric oxide (NO) has been mostly associated with so-called nitrosative stress or interaction with superoxide anion. However, recent investigations have indicated that, as for the other isoenzymes producing NO, guanylyl cyclase (GC) is a very sensitive target of iNOS activity. To further investigate this less explored signaling, the NO-cyclic guanosine 3â²-5â²-monophosphate (NO-cGMP)-induced vasodilator-stimulated phosphoprotein (VASP) phosphorylation on serine 239 was investigated in human embryonic kidney 293 cells (HEK cells). First, the expression and activity of α2 and β1 NO-sensitive GC subunits was determined by Western blot analysis, reverse transcription-polymerase chain reaction and NO donors administration. Then, the expression of a functional cGMP-dependent protein kinase I (PKGI) was verified by addition of 8-Br-cGMP followed by determination of phosphorylation of VASP on serine 239. Finally, iNOS activation of this signaling pathway was characterized after transfection of HEK cells with human iNOS cDNA. Altogether our data show that iNOS-derived NO activates endogenous NO-sensitive GC and leads to VASP phosphorylation in HEK cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Pharmacology - Volume 69, Issue 4, 15 February 2005, Pages 595-602
Journal: Biochemical Pharmacology - Volume 69, Issue 4, 15 February 2005, Pages 595-602
نویسندگان
Muriel André, Hélia Latado, Emanuela Felley-Bosco,