کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9011426 | 1124156 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effect of Gnaphalium conoideum HBK on guinea pig airway smooth muscle: role of L-type Ca2+ channels
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کلمات کلیدی
EGTAEC50LC50[Ca2+]i - [Ca2 +] iIntracellular Ca2+ - داخل سلولی Ca2 +Airway smooth muscle - عضله صاف راه هواییintracellular Ca2+ concentration - غلظت Ca2 + داخل سلولیeffective concentration 50% - غلظت موثر 50٪lethal concentration 50% - غلظت کشنده 50٪L-type Ca2+ channels - کانال Ca2 + L نوعMedicinal plants - گیاهان دارویی
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
داروشناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Plants from the Gnaphalium genus have been used in the Mexican traditional medicine for digestive and respiratory complaints. In the present study, the effect of methanolic extract from Gnaphalium conoideum HBK on the responses to contractile agonists was assessed in guinea pig tracheas, and the possible role of L-type Ca2+ channels was explored in tracheal guinea pig isolated myocytes. Cumulative concentration-response curves to carbachol or histamine, as well as contractile responses to 60 mM KCl were evaluated with or without 30 min preincubation with 20 or 100 μg mlâ1Gnaphalium conoideum. Likewise, intracellular Ca2+ concentrations were measured by microfluorometric method (fura-2 AM) in isolated tracheal myocytes with or without preincubation with 0.1, 0.31 or 1 μg mlâ1Gnaphalium conoideum. We found that methanolic extract from Gnaphalium conoideum significantly diminished the contractile responses to histamine, but not to carbachol or KCl. In isolated myocytes, Gnaphalium conoideum significantly reduced the intracellular Ca2+ rise induced by 60 mM KCl. Because histamine contractile responses are largely dependent on extracellular Ca2+, and KCl responses are mainly mediated through L-type Ca2+ channels, our results suggested that methanolic extract from Gnaphalium conoideum might be acting as a partial blocker of these Ca2+ channels.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Ethnopharmacology - Volume 97, Issue 2, 28 February 2005, Pages 267-272
Journal: Journal of Ethnopharmacology - Volume 97, Issue 2, 28 February 2005, Pages 267-272
نویسندگان
Patricia Campos-Bedolla, Luis M. Montaño, Edgar Flores-Soto, Abigail Aguilar, Ana MarÃa Puebla, Xavier Lozoya, Mario H. Vargas,