کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9012839 | 1125020 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Involvement of novel protein kinase C isoforms in carbachol-stimulated insulin secretion from rat pancreatic islets
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کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
کاردیولوژی و پزشکی قلب و عروق
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چکیده انگلیسی
The roles of protein kinase C (PKC) isoforms in cholinergic potentiation of glucose-induced insulin secretion were investigated in rat pancreatic islets. Western-blot analysis showed the presence of PKC-α, βII, δ, É, η, and ζ, but not PKC-βI, γ, or ι, in the islets. Carbachol (CCh) caused translocations of PKC-α, βII, δ, and É from the cytosol to the plasma membrane. CCh facilitated 7-mM glucose-induced insulin secretion from isolated rat islets. The CCh-stimulated insulin secretion was significantly suppressed by the generic PKC inhibitor chelerythrine. In contrast, Gö 6976, an inhibitor of conventional PKC isoforms, had no effect on the insulin secretion stimulated by CCh, although it significantly inhibited that induced by phorbol 12-myristate 13-acetate. These results suggest that the novel PKC isoforms activated by CCh, i.e., PKC-δ and/or É, participate in the stimulatory effect of CCh on insulin secretion.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Life Sciences - Volume 77, Issue 4, 10 June 2005, Pages 462-469
Journal: Life Sciences - Volume 77, Issue 4, 10 June 2005, Pages 462-469
نویسندگان
Tomohisa Ishikawa, Eri Iwasaki, Kazumitsu Kanatani, Fumi Sugino, Yukiko Kaneko, Kazuo Obara, Koichi Nakayama,