کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9017988 | 1128682 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Green tea polyphenol epigallocatechin-3-gallate differentially modulates oxidative stress in PC12 cell compartments
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کلمات کلیدی
N,N-dimethylnitrosamineDMNAGSHGSTCYP2E1EGCGPARPLPOCDNBEpigallocatechin-3-gallate3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide - 3- (4،5-Dimethylthiazol-2-yl) -2،5-diphenyltetrazoliumbromideMTT - MTTSDS-PAGE - الکتروفورز ژل پلی آکریل آمیدsodium dodecyl sulphate polyacrylamide gel electrophoresis - الکتروفورز ژل پلی اکریللید سدیم دودسیل سولفاتOxidative stress - تنش اکسیداتیوApoptosis - خزان یاختهایPC12 cells - سلول های PC12cytochrome P450 2E1 - سیتوکروم P450 2E1Lipid peroxidation - پراکسیداسیون لیپیدreduced glutathione - کاهش گلوتاتیونglutathione S-transferase - گلوتاتیون S-ترانسفراز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Tea polyphenols have been reported to be potent antioxidants and beneficial in oxidative stress related diseases. Prooxidant effects of tea polyphenols have also been reported in cell culture systems. In the present study, we have studied oxidative stress in the subcellular compartments of PC12 cells after treatment with different concentrations of the green tea polyphenol, epigallocatechin-3-gallate (EGCG). We have demonstrated that EGCG has differentially affected the production of reactive oxygen species (ROS), glutathione (GSH) metabolism and cytochrome P450 2E1 activity in the different subcellular compartments in PC12 cells. Our results have shown that although the cell survival was not inhibited by EGCG, there was, however, an increased DNA breakdown and activation of apoptotic markers, caspase 3 and poly- (ADP-ribose) polymerase (PARP) at higher concentrations of EGCG treatment. Our results suggest that the differential effects of EGCG might be related to the alterations in oxidative stress, GSH pools and CYP2E1 activity in different cellular compartments. These results may have implications in determining the chemopreventive therapeutic use of tea polyphenols in vivo.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology and Applied Pharmacology - Volume 207, Issue 3, 15 September 2005, Pages 212-220
Journal: Toxicology and Applied Pharmacology - Volume 207, Issue 3, 15 September 2005, Pages 212-220
نویسندگان
Haider Raza, Annie John,