کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9034835 | 1132649 | 2005 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Dose-dependent allergic responses to an extract of Penicillium chrysogenum in BALB/c mice
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
IL-5PBSRBLEMEMIgGMCHFCSIgEPCEHBSSPenicillium chrysogenumAUC - AUCAsthma - آسمAllergy - آلِرژیimmunoglobulin A - ایمونوگلوبولین AImmunoglobulin E - ایمونوگلوبولین Eimmunoglobulin G - ایمونوگلوبولین GIgA - ایمونوگلوبولین IgA Interleukin 5 - اینترلوکین 5BALF - بافتEnzyme-linked immunosorbent assay - تست الیزاELISA - تست الیزاfetal calf serum - سرم گوساله جنینPhosphate buffered saline - فسفات بافر شورRespiratory exposure - قرار گرفتن در معرض تنفسlactate dehydrogenase - لاکتات دهیدروژناز LDH - لاکتات دهیدروژناز به صورت مختصر شده LDH Rat basophil leukemia - لوسمی بازوفیل موش صحراییMaca - ماکاBronchoalveolar lavage fluid - مایع لارو برونکلوفلورMethacholine - متاکولینHank's balanced salt solution - محلول نمک متعادل هانکMouse model - مدل موشarea under the curve - منطقه تحت منحنی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Indoor mold has been associated with the development of allergic asthma. Penicillium chrysogenum, a common indoor mold, is known to have several allergens and can induce allergic responses in a mouse model of allergic penicilliosis. Our hypothesis is that soluble components of P. chrysogenum (PCE) can dose-dependently induce responses typical of allergic asthma in BALB/c mice. Mice were exposed to 10, 20, 50, or 70 μg of PCE by involuntary aspiration four times over a 4-week period. Serum and bronchoalveolar lavage fluid (BALF) were collected before (day 0), and at days 1 and 3 following the final exposure. PCE-exposed mice demonstrated dose-dependent increases in: BALF total cell numbers including eosinophil, serum and BALF total IgE levels, BALF IL-5 levels, and increased severity of histopathologic lesions. A single exposure to the highest dose of PCE resulted in edema and cellular damage but not immune responses. Four exposures to Metarhizium anisopliae crude antigen (10 μg, positive control) resulted in equivalent or greater allergic asthma-like responses than those demonstrated by multiple exposures to 50 or 70 μg of PCE. Multiple exposures to 70 μg of PCE showed increased allergen-triggered immediate respiratory responses as well as non-specific airway hyperresponsiveness to methacholine as assessed by barometric whole-body plethysmography. Taken together, repeated pulmonary challenge with P. chrysogenum extract induced dose-dependent allergic asthma-like responses in mice.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology - Volume 216, Issue 1, 1 December 2005, Pages 73-84
Journal: Toxicology - Volume 216, Issue 1, 1 December 2005, Pages 73-84
نویسندگان
Yong Joo Chung, Najwa Haykal Coates, Michael E. Viana, Lisa Copeland, Stephen J. Vesper, MaryJane K. Selgrade, Marsha D.W. Ward,