کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9038469 | 1133837 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Differential susceptibility of macrophage cell lines to Bacillus anthracis-Vollum 1B
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Differential susceptibility of macrophage cell lines to Bacillus anthracis-Vollum 1B Differential susceptibility of macrophage cell lines to Bacillus anthracis-Vollum 1B](/preview/png/9038469.png)
چکیده انگلیسی
Bacillus anthracis (BA) is a spore forming bacterium and the causative agent of anthrax disease. Macrophages (MÏs) play a central role in anthrax disease. An important step in disease progression is the ability of BA to secrete lethal toxin (LeTx) that kills MÏs. LeTx is a heterodimer composed of protective antigen (PA) and lethal factor (LF). Researchers have shown that MÏ cell lines demonstrate differential susceptibility to purified LeTx; for example RAW264.7 and J774A.1 MÏs are sensitive to LeTx whereas IC-21 MÏs are resistant. Research has also suggested that exogenous factors, including other BA proteins, can influence the activity of LeTx. For this reason, the objective of the current work was to examine if RAW264.7, J774A.1, and IC-21 MÏs demonstrated differential susceptibility when cultured with a LeTx-producing strain of BA. Here, we co-cultured MÏs with LeTx+ Vollum 1B (V1B) spores for >15Â h and assayed for MÏ cell death by morphology, trypan blue (TB) staining, neutral red (NR) activity, and lactate dehydrogenase (LDH) activity in the culture media. Following the addition of V1B spores, necrosis (â50% mortality) was observed in RAW264.7 and J774A.1 MÏs at 7.5 and 10Â h, respectively. By 15Â h, both RAW264.7 and J774A.1 MÏs demonstrated 100% mortality. In contrast, IC-21 MÏs, under identical culture conditions, remained viable (98%) and activated throughout the course of the experiment (>24Â h). The mechanism of RAW264.7 cell death appeared to involve LeTx because the V1B-induced cytotoxicity was dose-dependently reversed by the addition of anti-PA antibody to the culture media. These observations suggest there is differential susceptibility of MÏ cell lines to the LeTx+ V1B strain of BA. Further development of this in vitro model may be useful to further characterize the interactions between MÏs and BA spores.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 19, Issue 2, March 2005, Pages 221-229
Journal: Toxicology in Vitro - Volume 19, Issue 2, March 2005, Pages 221-229
نویسندگان
B.W. Gutting, K.S. Gaske, A.S. Schilling, A.F. Slaterbeck, L. Sobota, R.S. Mackie, T.L. Buhr,